Molecular mechanism of the anti-diabetic activity of an identified oligosaccharide from.
Res Pharm Sci. 2020 Feb ;15(1):36-47. Epub 2020 Feb 20. PMID: 32180815
Background and purpose: Because of the high prevalence, diabetes is considered a global health threat. Hence, the need for effective, cheap, and comfortable therapies are highly felt. In previous study, a novel oligosaccharide with strong anti-diabetic activity in the crude extract offruits, from thefamily, was identified. The present study was designed to ensure its efficacy usingandstudies.
Experimental approach: Crude extract and its purified oligosaccharide were prepared from corresponding herb. Adult male Wistar rats were randomly divided into four groups of 10 each, as follows: group 1, healthy control rats given only sterile normal saline; group 2, diabetic control rats received sterile normal saline; group 3, diabetic rats treated with crude extract of Rosa canina (40% w/v) by oral gavage for 8 weeks; group 4, diabetic rats treated with purified oligosaccharide of Rosa canina (2 mg/kg) by oral gavage for 8 weeks. After treatment, body weight, fasting blood glucose, serum insulin levels and islet beta-cell repair and proliferation were investigated. The possible cytoprotective action of oligosaccharide was evaluated. The effect of oligosaccharide on apoptosis and insulin secretion in cell culture media were examined. Real-time PCR was used to determine the expression level of some glucose metabolism-related regulator genes.
Findings / Results: In the animal model of diabetes, the insulin levels were increased significantly due to the regeneration of beta-cells in the islands of langerhans by the purified oligosaccharide.cell apoptosis examination showed that high concentration of oligosaccharide increased cell death, while at low concentration protected cells from streptozotocin-induced apoptosis. Molecular study showed that the expression ofandinsulin production genes were increased, leading to increased expression of insulin-dependent genes such asand. On the other hand, the expression of thegene, which is related to the glucose transporter 2, was significantly reduced due to insulin concentrations.
Conclusion and implications: The purified oligosaccharide fromwas a reliable anti-diabetic agent, which acted by increasing insulin production in beta-cells of the islands of Langerhans.