Mulberry cyanidin-3-glucoside-induced apoptosis in breast cancer cells. - GreenMedInfo Summary
Anti-cancer effect of cyanidin-3-glucoside from mulberry via caspase-3 cleavage and DNA fragmentation in vitro and in vivo.
Anticancer Agents Med Chem. 2017 Mar 27. Epub 2017 Mar 27. PMID: 28356020
Fruits of Morus alba L. (mulberry) have various bioactive compounds such as polyphenols and anthocyanins and used as a herbal medicine. However, the anti-cancer effects and molecular basis have not been elucidated. We examined the effect of the cyanidin-3-glucoside isolated from mulberry by acidified-methanol extraction on MDA-MB-453 human breast cancer cells. The results showed that treatment with the mulberry C3G increased cell death in a dose-dependent manner with alteration of apoptotic protein contents, and DNA fragmentation, suggesting that cells undergo apoptosis. Supporting the observations, treatment with the cyanidin-3-glucoside showed active apoptosis by caspase-3 cleavage and DNA fragmentation through Bcl-2 and Bax pathway. Indeed, cyanidin-3-glucoside inhibited tumor growth in MDA-MB-453 cells-inoculated nude mice. Tumor growth of xenograft nude mouse was reduced compared with the control group by the cyanidin-3-glucoside. These results show that mulberry cyanidin-3-glucoside inhibit the proliferation and growth in the in vitro and in vivo models. These data suggest that mulberry cyanidin-3-glucoside-induced apoptosis in breast cancer cells (MDA-MB-453), which has a potential to perform as an anti-cancer agent.