Neuroprotective effect ofagainst HO-induced PC12 cell cytotoxicity by reducing oxidative stress.
Food Sci Biotechnol. 2020 Nov ;29(11):1519-1530. Epub 2020 Aug 29. PMID: 33088601
Sang Ho Rho
In this study, the anti-oxidative and neuro-protective effects of ethanolic extracts of the dried roots ofwere investigated. Total phenolic contents and total flavonoid contents ofextract depended on the ethanol concentrations used (50, 70 and 95%). In order to evaluate radical scavenging activity, DPPH and ABTS radical scavenging assays and ferric reducing powers were evaluated. The results showed the 95% ethanol extract of(95AH) had higher phenolic and flavonoid contents, and greater radical scavenging activities than 50 or 70% ethanol extracts of. The neuro-protective effects of 95AH were evaluated using HO-treated PC12 neuronal cells. Treatment of 95AH increased cell viability and superoxide dismutase and glutathione peroxidase activities, reduced lactate dehydrogenate release, reduced reactive oxygen species production, and increased Bcl-2/Bax ratio. HPLC revealed 95AH was rich in phenolics, especially catechin. These results demonstrate 95AH has substantial anti-oxidative and neuro-protective effects against HO-induced oxidative stress.