Neuroprotective effect of apigenin against cerebral ischemia/reperfusion injury. - GreenMedInfo Summary
Neuroprotective effect of apigenin against cerebral ischemia/reperfusion injury.
J Int Med Res. 2020 Sep ;48(9):300060520945859. PMID: 32993408
OBJECTIVE: The therapeutic efficacy of apigenin in PC12 cells and rats remains uncertain. The aim of this study was to investigate the neuroprotective effects of apigenin against cerebral ischemia/reperfusion injury, bothand.
METHODS: We first treated PC12 cells with cobalt chloride (CoCl) to create a model of oxidative stress injury. Cell viability was then determined using a multifunctional microplate reader. In addition, reactive oxygen species (ROS) levels, apoptosis, and mitochondrial membrane potentials (MMPs) were examined using high-content cytometer analysis. The efficacy of apigenin treatment was also analyzed in a rat middle cerebral artery occlusion (MCAO) model using TTC staining and neurological deficit scores.
RESULTS: The half-inhibitory concentration of CoClwas 1.2 mM. Pretreatment with 10µg ⋅ mLapigenin significantly enhanced cell viability, reduced ROS levels, alleviated apoptosis, and improved MMP in PC12 cells with CoCl-induced injury. In addition, apigenin treatmentsignificantly improved neurological deficit scores and reduced infarct areas in MCAO rats. These results suggest that the neuroprotective mechanisms of apigenin may be related to mitochondrial activation.
CONCLUSIONS: Apigenin had excellent neuroprotective effects for the treatment of cerebral ischemia/reperfusion injuryand