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Abstract Title:

[Neuroprotective effects of paeonol in a cell model of Parkinson disease].

Abstract Source:

Zhejiang Da Xue Xue Bao Yi Xue Ban. 2015 Jan ;44(1):30-6. PMID: 25851972

Abstract Author(s):

Hao Wang, Zhao-Ming Geng, Zhi-Wei Hu, Shu-Yan Wang, Bing Zhao

Article Affiliation:

Hao Wang

Abstract:

OBJECTIVE: To investigate the effects of paeonol on neuron cell model of Parkinson disease (PD).

METHODS: The cell model of Parkinson disease was induced by treatment of 1-Methyl-4-phenylpyridinium (MPP+) in PC12 cells, the PD model cells were treated with 1μmol/L, 3 μmol/L or 9 μmol/L paeonol for 24h, respectively. Cell viability and LDH leakage were detected by MTT and lactate dehydrogenase (LDH) assay; the apoptosis of PC12 cells was assessed by Hoechst 33258 staining and flow cytometry; reactive oxygen species (ROS) production was detected by DCFH-DA method; and the ratio of Bax/Bcl-2 and activation of caspase-3 were determined by Western blotting.

RESULTS: MPP+ treatment significantly reduced cell viability, increased LDH leakage, enhanced the proportion of apoptotic cells and ROS production. In addition, MPP+ treatment dramatically increased the Bax/Bcl-2 ratio, and the activation of caspase-3. Compared to PD model group, paeonol treatment significantly enhanced cell viability, decreased LDH leakage, inhibited the proportion of apoptotic cells and ROS production, reduced the Bax/Bcl-2 ratio and the activated caspase-3 protein.

CONCLUSION: Paeonol can prevent PC12 cells from apoptosis induced by MPP+, and the mechanism may be associated with the down-regulation of ROS production, Bax/Bcl-2 ratio and Caspase-3 activation.

Study Type : In Vitro Study

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Sayer Ji
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