A novel Hericium erinaceus polysaccharide: Structural characterization and prevention of HO-induced oxidative damage in GES-1 cells.
Int J Biol Macromol. 2019 Nov 20. Epub 2019 Nov 20. PMID: 31759012
In this paper, the structural characteristics and bioactivity of a novel polysaccharide from H. erinaceus (HEP) were investigated, wherein, physico-chemical characterization demonstrated that HEPwith an average molecular weight of 12.713 kDa was composed of mannose (5.13%), glucose (43.02%), and galactose (51.85%). The models in vitro for preventing oxidative damage of human gastric epithelium (GES-1) cells were established and used to investigate the preventive effects of HEPfrom oxidative damage. It was found that HEPcould significantly prevent GES-1 cells against HO-induced oxidative damage by promoting cell proliferation, inhibiting cell necrosis, reducing ROS levels, regulating mitochondrial membrane potential and maintaining mitochondrial membrane permeability. These results indicated HEPcan effectively prevent gastric cell damage in vitro and suggested the potential application of HEPas bioactive ingredient for healthy foods.