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Abstract Title:

The oil-resin of the tropical rainforest tree Copaifera langsdorffii reduces cell viability, changes cell morphology and induces cell death in human endometriotic stromal cultures.

Abstract Source:

J Pharm Pharmacol. 2015 Dec ;67(12):1744-55. Epub 2015 Sep 26. PMID: 26407531

Abstract Author(s):

Julianna Henriques da Silva, Vinicius Raphael de Almeida Borges, Leonardo da Cunha Boldrini Pereira, Renato Ferrari, Rômulo Medina de Mattos, Eliane Gouveia de Oliveira Barros, Celia Yelimar Palmero, Patricia Dias Fernandes, Patricia Ribeiro de Carvalho, Valeria Pereira de Sousa, Lucio Mendes Cabral, Luiz Eurico Nasciutti

Article Affiliation:

Julianna Henriques da Silva

Abstract:

OBJECTIVES: The hormonal treatment for endometriosis frequently fails to completely eradicate endometriotic implants. A new therapeutic treatment is needed. This study investigates the in-vitro effect of Copaifera langsdorffii oil-resin on human eutopic and ectopic endometrium stromal cell cultures (EuESCs and EctESCs).

METHODS: A nanocomposite system containing the copaiba oil-resin (NanoCOR) was developed and acute toxicity test was performed. Endometrial stromal cells (ESCs) from non-endometriotics controls (CESCs), EuESCs and EctESCs were isolated and treated with different concentrations of NanoCOR, at different time intervals to evaluate its effect on cell morphology, proliferation, viability, necrosis and apoptosis induction.

KEY FINDINGS: When treated with 50 μg/ml of NanoCOR, the morphology of EctESCs changed, as the actin microfilaments were disorganized, disassembled or disrupted. Moreover, at 24 h of treatment with NanoCOR, the EctESCs viability was inhibited, and a significant number of these cells underwent apoptosis. In EuESCs, these effectswere observed only at 48 h. Finally, the treatment of EctESCs with NanoCOR increased the lactate dehydrogenase release into the extracellular medium more than in EuESCs.

CONCLUSIONS: Our data indicate that NanoCOR has a greater impact on the behaviour of human endometriotic stromal cells than on the eutopic endometrium stromal cells, supporting the idea that NanoCOR should be further investigated as a novel and valuable alternative to treat endometriosis.

Study Type : In Vitro Study

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Sayer Ji
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