Aronia (Aronia melanocarpa) Polyphenols Modulate Microbial Community in Simulator of the Human Intestinal Microbial Ecosystem (SHIME) and Decrease Secretion of Pro-Inflammatory Markers in a Caco-2/endothelial Cell Co-Culture Model.
Mol Nutr Food Res. 2018 Sep 19:e1800607. Epub 2018 Sep 19. PMID: 30231187
SCOPE: To explore the mechanisms behind the health effects of Aronia (Aronia melanocarpa), the microbial community modulating and anti-inflammatory effects of Aronia polyphenols were investigated by combining SHIMEwith a co-culture of intestinal and endothelial cells.
RESULTS: Administration of Aronia juice (6.5g/L) to the SHIMEfor 2 weeks increased the abundance of Firmicutes to 92% in AC, 85% in TC and 82% in DC(p < 0.001), Proteobacteria(6.7% in AC, p < 0.001) and Akkermansia (14% in TC and 18% in DC, p < 0.001) and decreased the abundance of Bifidobacterium species, associated with a decrease of acetate and increase of propionate and butyrate, whereas no significant difference was observed upon placebo juice treatment. After addition of the digests to TNF-α challenged Caco-2/endothelial co-cultures, ICAM-1, IL-8 and MCP-1 levels were significantly downregulated. Interestingly, Aronia juice treated digests from each colon compartments resulted in a stronger decrease of the ICAM-1 secretion (up to 73%, p < 0.001) compared to their corresponding placebo treated digests, thereby pointing to a polyphenol-dependent effect.
CONCLUSIONS: Aronia polyphenols modulate intestinal microbial composition, induce beneficial SCFA production and prevent inflammatory stress in endothelial cells. This opens perspectives for the use of Aronia polyphenols as prebiotics in the context of intestinal and cardiovascular health. This article is protected by copyright. All rights reserved.