Inhibits Cell Wall Synthesis and Triggers Membrane Depolarization in.
J Microbiol Biotechnol. 2017 Feb 28 ;27(2):395-404. PMID: 28100900
Fungal cell walls and cell membranes are the main targets of antifungals. In this study, we report on the antifungal activity of an ethanol extract fromagainst, showing that the antifungal activity is associated with the synergistic actions of preventing cell wall synthesis, enabling membrane depolarization, and compromising permeability. First, it was shown that the ethanol extract fromwas involved in damaging the integrity of cell walls in. In isotonic media, cell bursts ofby theethanol extract could be restored, and the minimum inhibitory concentration (MIC) of theethanol extract againstcells increased 4-fold. In addition, synthesis of (1,3)-β--glucan polymer was inhibited by 87% and 83% following treatment ofmicrosomes with theethanol extract at their 1× MIC and 2× MIC, respectively. Second, the ethanol extract frominfluenced the function ofcell membranes.cells treated with theethanol extract formed red aggregates by staining with a membrane-impermeable dye, propidium iodide. Membrane depolarization manifested as increased fluorescence intensity by staining-treatedcells with a membrane-potential marker, DiBAC(3) ((-1,3-dibutylbarbituric acid) trimethine oxonol). Membrane permeability was assessed by crystal violet assay, andcells treated with theethanol extract exhibited significant uptake of crystal violet in a concentration-dependent manner. The findings suggest thatethanol extract is a viable and effective candidate for the development of new antifungal agents to treat-associated diseases.