Paeonol attenuates quorum-sensing regulated virulence and biofilm formation in Pseudomonas aeruginosa. - GreenMedInfo Summary

With the prevalence of multidrug-resistant bacteria and clinical -acquired pathogenic infections, the development of quorum-sensing (QS) interfering agents is one of the most potential strategies to combat bacterial infections and antibiotic resistance. Chinese herbal medicines constitute a valuable bank of resources for the identification of QS inhibitors. Accordingly, in this research, some compounds were tested for QS inhibition using indicator strains. Paeonol is a phenolic compound, which can effectively reduce the production of violacein without affecting its growth inATCC 12472, indicating its excellent anti-QS activity. This study assessed the anti-biofilm activity of paeonol against Gram-negative pathogens and investigated the effect of paeonol on QS-regulated virulence factors in. Ainfection model was used to explore the anti-infection ability of paeonol. Paeonol exhibited an effective anti-biofilm activity against Gram-negative bacteria. The ability of paeonol to interfere with the AHL-mediated quorum sensing systems ofwas determined, found that it could attenuate biofilm formation, and synthesis of pyocyanin, protease, elastase, motility, and AHL signaling molecule in a concentration- and time-dependent manner. Moreover, paeonol could significantly downregulate the transcription level of the QS-related genes ofincluding, as well as mediated its virulence factors,, and.studies revealed that paeonol could reduce the pathogenicity ofand enhance the survival rate of, showing a moderate protective effect on. Collectively, these findings suggest that paeonol attenuates bacterial virulence and infection ofand that further research elucidating the anti-QS mechanism of this compoundis warranted.