Parthenolide reduces gene transcription of prosurvival mediators in U937 cells. - GreenMedInfo Summary
Parthenolide reduces gene transcription of prosurvival mediators in U937 cells.
Exp Oncol. 2017 03 ;39(1):30-35. PMID: 28361855
: In acute myeloid leukemia (AML) the functional abnormalities of osteopontin (OPN), NF-kB, PI3K/AKT/mTOR/PTEN pathway orβ-catenin have been considered.
AIM: To analyze the response of U937 cells to parthenolide (PTL) through the involvement of expression of OPN protein, RelB, AKT1, mTOR, PTEN andβ-catenin genes.
MATERIALS AND METHODS: The U937 cells were treated with PTL at concentrations of 4μM (IC25) or 6 μM (IC50) and with OPN siRNA for MTT assay and colony forming assay. Western blot analysis using antibodies against OPN was performed with lysates of PTL-treated cells. Quantitative real-time polymerase chain reaction was performed using primers for OPN siRNA, RelB, AKT1, mTOR, PTENand β-catenin.
RESULTS: PTL reduces OPN protein level and down-regulates RelB mRNA in U937 cell line. Suppression of OPN with siRNA increases the cytotoxic effects of PTL. Also, mRNA expression of AKT1, mTOR, PTEN, andβ-catenin decreases with PTL or OPN siRNA.
CONCLUSION: Sensitivity of U937 cells to PTL can be associated with the reduction in expression of prosurvival mediators.