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Abstract Title:

Petatewalide B, a novel compound from Petasites japonicus with anti-allergic activity.

Abstract Source:

J Ethnopharmacol. 2016 Feb 3 ;178:17-24. Epub 2015 Dec 7. PMID: 26674157

Abstract Author(s):

Young-Whan Choi, Kyoung-Pil Lee, Jung-Min Kim, Saeromi Kang, Soo-Jin Park, Jung-Min Lee, Hyung Ryong Moon, Jee H Jung, Young-Geun Lee, Dong-Soon Im

Article Affiliation:

Young-Whan Choi

Abstract:

ETHNOPHARMACOLOGICAL RELEVANCE: The giant butterbur Petasites japonicus is used to treat asthma and allergic diseases in traditional Korean, Japanese, and Chinese medicine.

AIM OF THE STUDY: To elucidate the anti-allergic effect of Petasites genus, we studied effects of several compounds from Petasites japonicus leaves and found a novel bakkenolide-type sesquiterpine. In the present study, anti-allergic and anti-inflammatory effects of the new compound was examined using in vivo and in vitro experiments.

MATERIALS AND METHODS: The novel compound was isolated from Petasites japonicus leaves and named petatewalide B. Antigen-induced degranulation and Ca(2+) mobilization were measured in RBL-2H3 mast cells by measuringβ-hexosaminidase activity and fluorescence change of Ca(2+) probe, fura-2. Induction of inducible nitric oxide synthase and cyclooxygenase 2 was measured by Western blotting in peritoneal macrophages. In addition, ovalbumin-induced asthma model was used for in vivo efficacy test of petatewalide B.Membrane potential was estimated by measuring fluorescence change of DiBAC in C6 glioma cells.

RESULTS: Petatewalide B inhibited the antigen-induced degranulation ofβ-hexosaminidase in RBL-2H3 mast cells, but did not affect antigen-induced Ca(2+) increase in the cells. Petatewalide B also showed inhibition of the LPS-induced induction of iNOS, but not COX-2 in mouse peritoneal macrophages. Nitric oxide production was also inhibited by petatewalide B in macrophages. In the ovalbumin-induced asthma model, petatewalide B strongly inhibited accumulations of eosinophils, macrophages, and lymphocytes in bronchoalveolar lavage fluid. Petatewalide B increased the membrane potential of C6 glioma cells in a concentration-dependent manner.

CONCLUSION: Petatewalide B from Petasites genus not only has anti-allergic and anti-inflammatory effects but also induces a transient increase of membrane potential in C6 glioma cells.

Study Type : In Vitro Study

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