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Abstract Title:

Photobiomodulation therapy compensate the impairments of diabetic bone marrow mesenchymal stem cells.

Abstract Source:

Lasers Med Sci. 2019 Jul 23. Epub 2019 Jul 23. PMID: 31338628

Abstract Author(s):

Fatemeh Zare, Mohammad Bayat, Abbas Aliaghaei, Abbas Piryaei

Article Affiliation:

Fatemeh Zare

Abstract:

Pathophysiologic conditions associated with diabetes mellitus affect mesenchymal stem cells (MSCs), and this phenomenon may lead to some diabetic secondary complications. The present study was conducted to evaluate the impact of photobiomodulation (PBM) on rat diabetic MSC (DMSC) behavior in vitro. For the purpose of PBM, we used helium-neon laser with a wavelength of 632.8 nm at three different energy densities (0.5, 1, 2 J/cm) and radiation periodicity of once, twice, and thrice. The survival, proliferation, and apoptosis in the normal MSCs (NMSCs), DMSCs, and diabetic MSCs, which were laser irradiated (DMSCs+L), were assessed using MTT assay, Ki67 immunofluorescence staining, and TUNEL assay, respectively. Our results demonstrated that DMSCs have significantly lower survival (P<0.05) and proliferation rates (P<0.001), and dramatically higher population doubling time (PDT, P<0.001) and apoptosis rates (P<0.001) as compared to NMSCs. Moreover, PBM with energy density of 1 J/cmand the periodicity of 1 or 2 times could improve diabetic MSC capabilities in the term of survival, proliferation, and apoptosis. Considering these findings, it is suggested that PBM could improve the ability of diabetic MSCs in vitro prior to transplantation or may rise their capabilities in their native niche in vivo.

Study Type : Animal Study, In Vitro Study

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