HIV-1 inhibitory compounds from Calophyllum brasiliense leaves.
Biol Pharm Bull. 2004 Sep;27(9):1471-5. PMID: 15340243
Department of Natural Products, Institute of Chemistry, National University of Mexico, Ciudad Universitaria, 04510, Mexico D. F., Mexico.
The hexane, acetone and methanol extracts of Calophyllum brasiliense leaves were fractionated following a three bioassay guide: high HIV-1 RT inhibition, low cytotoxicity on MT2 cells and high inhibition of HIV-1 IIIb/LAV replication. This led to the isolation of three anti HIV-1 dipyranocoumarins: calanolides A and B and soulattrolide. In contrast, other isolated compounds such as apetalic acid, isoapetalic acid, a structural isomer of isoapetalic acid, friedelin, canophyllol and amentoflavone were devoid of HIV-1 RT inhibitory activity. Calanolide C was also obtained as a natural product and showed moderate inhibitory properties.