Inhibition ofBiofilms by the Natural Stilbene Piceatannol Through the Inhibition of Glucosyltransferases.
ACS Omega. 2018 Jul 31 ;3(7):8378-8385. Epub 2018 Jul 30. PMID: 30087944
Removal of oral biofilms involves the use of broad-spectrum antimicrobials, which eradicate both pathogenic and protective oral commensal species. Ideal therapeutics for dental caries should be able to selectively inhibit pathogenic biofilms caused by.extracellular glucosyltransferases (Gtfs), particularly GtfB and GtfC, synthesize predominantly water-insoluble glucans, which contribute to the structural scaffold of biofilms. The lead stilbene identified through our docking study against the catalytic domain of GtfC is a natural product known as piceatannol, which inhibitedbiofilm formation in a dose-dependent manner, with considerable selectivity over growth inhibition ofand commensal streptococci. Binding kinetic analysis of piceatannol was performed using Octet RED against both GtfB and GtfC, which produced low micromolarvalues. Piceatannol inhibitedcolonization in an in vivo drosophila model and a rat model of dental caries.