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Article Publish Status: FREE
Abstract Title:

Piceatannol Increases Antioxidant Defense and Reduces Cell Death in Human Periodontal Ligament Fibroblast under Oxidative Stress.

Abstract Source:

Antioxidants (Basel). 2019 Dec 23 ;9(1). Epub 2019 Dec 23. PMID: 31878036

Abstract Author(s):

Flávia Póvoa da Costa, Bruna Puty, Lygia S Nogueira, Geovanni Pereira Mitre, Sávio Monteiro Dos Santos, Bruno José Brito Teixeira, Maria Sueli da Silva Kataoka, Manoela Domingues Martins, Carlos Augusto Galvão Barboza, Marta Chagas Monteiro, Hervé Rogez, Edivaldo Herculano Corrêa de Oliveira, Rafael Rodrigues Lima

Article Affiliation:

Flávia Póvoa da Costa

Abstract:

Piceatannol is a resveratrol metabolite that is considered a potent antioxidant and cytoprotector because of its high capacity to chelate/sequester reactive oxygen species. In pathogenesis of periodontal diseases, the imbalance of reactive oxygen species is closely related to the disorder in the cells and may cause changes in cellular metabolism and mitochondrial activity, which is implicated in oxidative stress status or even in cell death. In this way, this study aimed to evaluate piceatannol as cytoprotector in culture of human periodontal ligament fibroblasts through in vitro analyses of cell viability and oxidative stress parameters after oxidative stress induced as an injury simulator. Fibroblasts were seeded and divided into the following study groups: control, vehicle, control piceatannol, HOexposure, and HOexposure combined with the maintenance in piceatannol ranging from 0.1 to 20μM. The parameters analyzed following exposure were cell viability by trypan blue exclusion test, general metabolism status by the 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) method, mitochondrial activity through the ATP production, total antioxidant capacity, and reduced gluthatione. Piceatannol was shown to be cytoprotective due the maintenance of cell viability between 1 and 10 μM even in the presence of HO. In a concentration of 0.1μM piceatannol decreased significantly cell viability but increased cellular metabolism and antioxidant capacity of the fibroblasts. On the other hand, the fibroblasts treated with piceatannol at 1 μM presented low metabolism and antioxidant capacity. However, piceatannol did not protect cells from mitochondrial damage as measured by ATP production. In summary, piceatannol is a potent antioxidant in low concentrations with cytoprotective capacity, but it does not prevent all damage caused by hydrogen peroxide.

Study Type : In Vitro Study

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Sayer Ji
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