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Abstract Title:

Piperlongumine Induces Apoptosis in Colorectal Cancer HCT 116 Cells Independent of Bax, p21 and p53 Status.

Abstract Source:

Anticancer Res. 2018 Nov ;38(11):6231-6236. PMID: 30396942

Abstract Author(s):

Fernanda DA Silva Machado, Fernanda Mosena Munari, Fernando Joel Scariot, Sergio Echeverrigaray, Cesar Aguzzoli, Claus Tröger Pich, Massuo Jorge Kato, Lydia Yamaguchi, Sidnei Moura, João Antonio Pêgas Henriques, Mariana Roesch-Ely

Article Affiliation:

Fernanda DA Silva Machado

Abstract:

BACKGROUND/AIM: Colorectal cancer is a common type of cancer with reported resistance to treatment, in most cases due to loss of function of apoptotic and cell-cycle proteins. Piperlongumine (PPLGM) is a natural alkaloid isolated from Piper species, with promising anti-cancer properties. This study investigated whether PPLGM is able to induce cell death in colorectal carcinoma HCT 116 cells expressing wild-type or deficient in Bax, p21 or p53.

MATERIALS AND METHODS: PPLGM was extracted from roots of Piper tuberculatum. Cell viability was determined by reduction of 3-(4,5-dimethilthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and clonogenic assay. Cell death was evaluated by acridine orange/ethidium bromide staining and flow cytometry. Plasmid cleavage activity and circular dichroism DNA interaction were also analyzed.

RESULTS: PPLGM induced selective cell death in all cell lines (ICrange from 10.7 to 13.9μM) with an increase in the number of late apoptotic cells and different profiles in cell-cycle distribution. Plasmid DNA analysis showed that PPLGM does not interact directly with DNA.

CONCLUSION: This paper suggests that PPLGM may be a promising candidate in colorectal cancer therapy.

Study Type : In Vitro Study

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