Placenta-derived stem cells have anti-proliferative properties against cancer cell lines. - GreenMedInfo Summary
Amniotic Membrane-Derived Cells Inhibit Proliferation of Cancer Cell Lines by Inducing Cell Cycle Arrest.
J Cell Mol Med. 2012 Jan 19. Epub 2012 Jan 19. PMID: 22260183
Centro di Ricerca E. Menni, Fondazione Poliambulanza Istituto Ospedaliero, 25124, Brescia, Italy.
Cells derived from the amniotic fetal membrane of human term placenta have drawn particular attention mainly for their plasticity and immunological properties, which render them interesting for stem cell research and cell-based therapeutic applications. In particular, we have previously demonstrated that amniotic mesenchymal tissue cells (AMTC) inhibit lymphocyte proliferation in vitro and suppress the generation and maturation of monocyte-derived dendritic cells. Here we show that AMTC also significantly reduce the proliferation of cancer cell lines of hematopoietic and non-hematopoietic origin, in both cell-cell contact and transwell co-cultures, therefore suggesting the involvement of yet unknown inhibitory soluble factor(s) in this"cell growth restraint". Importantly, we provide evidence that the anti-proliferative effect of AMTC is associated to induction of cell cycle arrest in G0/G1 phase. Gene expression analyses demonstrate that AMTC can down-regulate cancer cells' mRNA expression of genes associated with cell cycle progression, such as cyclins (cyclin D2, cyclin E1, cyclin H) and cyclin-dependent kinase (CDK4, CDK6 and CDK2), whilst they up-regulate cell cycle negative regulator such as p15 and p21, consistent with a block in G0/G1 phase with no progression to S phase. Taken together, these findings warrant further studies to investigate the applicability of these cells for controlling cancer cell proliferation in vivo.© 2012 The Authors Journal of Cellular and Molecular Medicine © 2012 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd.