Abstract Title:

A plant viral"reinitiation"factor interacts with the host translational machinery.

Abstract Source:

Cell. 2001 Sep 21 ;106(6):723-33. PMID: 11572778

Abstract Author(s):

H S Park, A Himmelbach, K S Browning, T Hohn, L A Ryabova

Article Affiliation:

Friedrich Miescher-Institute, P.O. Box 2543, CH-4002, Basel, Switzerland.

Abstract:

The cauliflower mosaic virus transactivator, TAV, controls translation reinitiation of major open reading frames on polycistronic RNA. We show here that TAV function depends on its association with polysomes and eukaryotic initiation factor eIF3 in vitro and in vivo. TAV physically interacts with eIF3 and the 60S ribosomal subunit. Two proteins mediating these interactions were identified: eIF3g and 60S ribosomal protein L24. Transient expression of eIF3g and L24 in plant protoplasts strongly affects TAV-mediated reinitiation activity. We demonstrate that TAV/eIF3/40S and eIF3/TAV/60S ternary complexes form in vitro, and propose that TAV mediates efficient recruitment of eIF3 to polysomes, allowing translation of polycistronic mRNAs by reinitiation, overcoming the normal cell barriers to this process.

Study Type : Viral

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