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Abstract Title:

Purification, characterization and neuroprotective effects of a polysaccharide from Gynostemma pentaphyllum.

Abstract Source:

Carbohydr Polym. 2015 May 20 ;122:93-100. Epub 2014 Dec 31. PMID: 25817647

Abstract Author(s):

Dong Jia, Chunguang Rao, Shengxiang Xue, Jinli Lei

Article Affiliation:

Dong Jia

Abstract:

In the present study, we investigated the neuroprotective effects of GPP1, a purified polysaccharide from Gynostemma pentaphyllum, on Aβ (25-35)-induced neuronal toxicity and explore its potential mechanisms in PC12 cells. The results showed pretreatment with GPP1 prior to Aβ (25-35) exposure significantly protected PC12 cells from Aβ (25-35)-induced cell death, lactate dehydrogenase (LDH) release, DNA damage, intracellular freecalcium concentration ([Ca(2+)]i) influx, mitochondrial dysfunction and mitochondrial cytochrome c (Cyt-C) release. In addition, pretreatment with GPP1 also protected PC12 cells against Aβ (25-35)-induced the accumulation of reactive oxygen species (ROS) and lipid peroxidation product MDA, the reduction in glutathione (GSH) level and superoxide dismutase (SOD) activity, the increased Bax/Bcl-2 protein expression ratio and caspase-3 activation. Our findings suggest that GPP1 exerts a neuroprotective effect against Aβ (25-35)-induced neurotoxicity in PC12 cells, at least in part, via inhibiting oxidative stress and suppressing the mitochondrial apoptotic pathway.

Study Type : In Vitro Study

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