Pomegranate peel extract decreases small intestine lipid peroxidation by enhancing activities of major antioxidant enzymes.
J Sci Food Agric. 2015 Nov 13. Epub 2015 Nov 13. PMID: 26564426
Kaïs H Al-Gubory
BACKGROUND: Pomegranate peel extract (PPE) contains several compounds with antioxidative properties. Addition of PPE to foods may interact with endogenous antioxidants and promote health outcome. However, little is known about the biochemical mechanisms by which PPE exert their actions on tissues of biological systems in vivo. The purpose of this study was to determine the effects of PPE on activities of antioxidant enzymes. Mice were used to investigate the effects of PPE on plasma levels of malondialdehyde (MDA), tissue MDA content and activities of superoxide dismutase 1 (SOD1), SOD2 and glutathione peroxidase (GPX) in the small intestine, liver and skeletal muscle, different tissues involved in the digestion, absorption and metabolism of dietary nutrients. Control mice were fed with a standard diet, while treated mice were fed for 40 days the standard diet containing 5% or 10% PPE.
RESULTS: Mice fed the 10% PPE diet exhibited lower plasma MDA concentrations, reduced contents of MDA in the small intestine and liver and higher levels of SOD1 and GPX activities in the small intestine compared to the mice fed the control diet.
CONCLUSIONS: These findings demonstrates that intake of PPE in diet attenuates small intestine lipid peroxidation and strengthens the first line of small intestine antioxidant defenses by enhancing enzymatic antioxidative pathways. PPE is worthy of further study as a therapeutic approach to prevent peroxidative stress-induced gut pathogenesis.