Protective effect of pomegranate seed oil on hexachlorobutadiene-induced nephrotoxicity in rat kidneys.
Ren Fail. 2010 Jun;32(5):612-7. PMID: 20486845
Department of Pharmacology and Pharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, 9177948564 Mashhad, I. R. Iran. firstname.lastname@example.org
Hexachlorobutadiene (HCBD) is a potent nephrotoxin in rodents. Pharmacological studies have shown that pomegranate fruit preparations have antioxidant, anti-inflammatory chemopreventive effects. In this study, the effect of pomegranate seed oil (PSO) on HCBD-induced nephrotoxicity was investigated in adult male rats. Animals were divided into five groups. Group 1 was treated with corn oil (1 mL/kg, i.p.). Group 2 received a single dose of HCBD (50 mg/kg, i.p.). Groups 3-5 were treated with PSO (0.16, 0.32, and 0.64 mg/kg, i.p., respectively) 1 h before HCBD (50 mg/kg, i.p.) injection. A significant elevation of serum creatinine and urea (p<0.001) levels as well as urine glucose and protein (p<0.001) concentrations (as markers of acute renal failure) was observed 24 h after administration of HCBD as compared to control group. HCBD also caused a significant decrease in total thiol content (p<0.001) and a significant increase in thiobarbituric acid reactive species (TBARS, as an index of lipid peroxidation) levels (p<0.001) in kidney homogenate samples. PSO pretreatment resulted in a significant and dose-dependent decrease in serum creatinine (p<0.001) and urea levels (p<0.001) as well as urine glucose (p<0.001) and protein concentrations (p<0.001) when compared with HCBD treated alone. PSO also significantly reversed the HCBD-induced depletion in total thiol content (p<0.001) and elevation in TBARS (p<0.001) in kidney homogenate samples. The results of this study showed that PSO clearly attenuated HCBD-induced nephrotoxicity, but explanation and mechanism of this protection need further explorations.