Pre-Treatment with Grape Seed Extract Reduces Inflammatory Response and Oxidative Stress Induced byInfection in Human Gastric Epithelial Cells.
Antioxidants (Basel). 2021 Jun 11 ;10(6). Epub 2021 Jun 11. PMID: 34208004
Jose Manuel Silvan
() is a pathogenic bacteria identified as a potential risk factor for gastritis, gastric ulcers and gastric cancer. During the stomach colonization,triggers a strong inflammatory response and subsequent oxidative stress, which are associated with tissue damage. For this reason, it is of particular interest to develop alternative natural tools that enable modulation of the associated damaging immune response. With this purpose, we obtained grape seed extract (GSE) from sweet (not fermented) food grade seeds. The aim of our study was to investigate the effect of GSE and its two enriched procyanidins fractions (OPC and PPC) on the inflammatory process and oxidative stress produced by differentstrains in human gastric epithelial cells (AGS). Anti-inflammatory activity was evaluated by measuring the level of interleukin-8 (IL-8) secretion. IL-8 production was significantly reduced in-infected human gastric epithelial cells pre-treated with GSE or its enriched fractions when compared with non-pre-treated infected cells (from 21.6% to 87.8%). Pre-treatment with GSE or its fractions significantly decreased intracellular reactive oxygen species (ROS) production in AGS cells after infection, depending on thestrain. Our results also showed that GSE and its fractions demonstrate antibacterial activity against all strains ofused in the study. This work demonstrates the effectiveness of GSE enriched in procyanidins against the main events associated withinfection.