Brazil nut consumption modulates Nrf2 expression in hemodialysis patients: A Pilot Study.
Mol Nutr Food Res. 2016 Mar 15. Epub 2016 Mar 15. PMID: 26992136
Ludmila F M F Cardozo
SCOPE: Previous studies showed that the supplementation with Brazil nut is effective in improving Se status, reduce oxidative stress and inflammation in hemodialysis (HD) patients. As the nutritional compounds may promote the reduction of inflammation by activation of nuclear factor E2-related factor 2 (Nrf2), the aim of this pilot study was to evaluate the effects of Brazil nut supplementation on Nrf2 activation in HD patients.
METHODS AND RESULTS: Thirteen HD patients received one Brazil nut supplementation per day for 3 months and were compared to twelve HD patients without supplementation. Peripheral blood mononuclear cells (PBMC) were isolated and processed for expression of nuclear factor kappa B (NF-κB), Nrf2 and quinoneoxidoreductase 1 (NQO1) by quantitative real-time polymerase chain reaction. Plasma malondialdehyde (MDA), C-reactive protein (CRP) and IL-6 levels were measured before and after supplementation. The Nrf2 expression increased and NF-κB expression reducedpost supplementation. In addition, the cytokines and MDA levels decreased significantly. No significant alterations were found in the control group.
CONCLUSION: This preliminary result indicates the effectiveness of Brazil nut supplementation on human Nrf2 activation in HD patients and could be a possible explanation for the beneficial effects of this nut as a bioactive compound. This article is protected by copyright. All rights reserved.