Inhibition of Lung Cancer Proliferation by Wogonin is Associated with the Activation of Apoptosis and Generation of Reactive Oxygen Species
Balkan Med J. 2019 Oct 9. Epub 2019 Oct 9. PMID: 31594288
Background: Lung cancer has very high incidence rate and is one among the commonly diagnosed tumors in the developed countries.
Aims: The present study was designed to investigate the effect of wogonin on A549 and A427 lung cancer cells and explore the mechanism involved.
Study Design: In the present study effect of wogonin was investigatedon A549 and A427 lung cancer and BEAS-2B normal cell normal lung cells.
Methods: The cytotoxicity effect of wogoninon A549 and A427 lung cancer and BEAS-2B cells was assessed by MTT assay. The apoptosis onset was assessed by flow cytometry using Annexin V FITC/PI staining. Western blotting was used for determination of changes in apoptotic protein expression.
Results: Wogonin treatment exhibited cytotoxicity effect selectively on A549 and A427 cells without affecting BEAS-2B normal lung cells. The viability of A549 and A427 cells was reduced to 31 and 34%, respectively on treatment with 50µM of wogonin. There was no significant reduction in BEAS-2B cell viability on treatment with 50 µM of wogonin. The percentage of apoptotic A427 cells showed a significant (P<0.049) increase on treatment with wogonin. Treatment of A427 cells with wogonin led to a marked increase in the activation of caspase 3/ 8/ 9. Wogonin treatment of A427 cells caused a marked increase in the production of reactive oxygen species at 72 h. Digital tomosynthesis (DTS) studies showed marked reduction in tumor development on treatment with wogonin.
Conclusion: In summary, present study demonstrated that wogonin treatment specifically exhibits cytotoxicity effect on lung cancer cells. Moreover, the cytotoxicity effect of wogonin is associated with the activation of apoptosis and generation of ROS. Therefore, wogonin can be developed for the treatment of lung cancer.