Inhibits Growth, Biofilm Formation, and Virulence Factor Expression of.
J Med Food. 2019 Jun ;22(6):623-630. Epub 2019 Apr 25. PMID: 31021282
This study was designed to determine whether the ethanol extract ofcould inhibit the cariogenic activity of. The increase in acid production and biofilm formation bywere evaluated. The expression levels of virulence factor genes were determined by performing the real-time polymerase chain reaction (PCR). The bactericidal effect was tested by confocal laser scanning microscopy. Theextract was observed to inhibit the growth ofat concentrations>0.05 mg/mL ( < .05). After using the safranin staining method, we found that theextract had an inhibitory effect against biofilm formation at a concentration of>0.05 mg/mL. These experimental results were similar to that observed with the scanning electron microscopy. The results of the confocal microscopy revealed that theextract at high concentrations of 0.4-3.2 mg/mL showed a bactericidal effect in a concentration-dependent manner. According to the results of the real-time PCR analysis, it was observed that theextract inhibited the expression of virulence factor genes. These results suggest thatmay inhibit the cariogenic activity of, and may be useful as an anticariogenic agent.