ProbioticGG Promotes Mouse Gut Microbiota Diversity and T Cell Differentiation.
Front Microbiol. 2020 ;11:607735. Epub 2020 Dec 17. PMID: 33391230
Lactic acid bacteria (LAB) are the primary genera of the intestinal flora and have many probiotic functions. In the present study,(LGG) ATCC 53103 was used to treat BALB/c mice. After LGG intervention, both low and high LGG doses were shown to improve the observed OTU, Chao1, ACE, and Shannon indices, while the Simpson index decreased, demonstrating that LGG can promote intestinal microbiota abundance and diversity. Furthermore, LGG treatment increased the abundances of intestinal,andwhile reducing that of. In addition to its effect on gut the microbiota, LGG could also regulate the host immune system. In the present study, we showed that LGG could affect the percentage of CD3T lymphocytes in the spleens (SPLs), mesenteric lymph nodes (MLNs), Peyer's patches (PPs) and lamina propria lymphocytes (LPLs) of mice, including total CD3T, CD3CD4T, and CD3CD8T lymphocytes. Furthermore, LGG could effectively increase the expression of Th1-type cytokines (IFN-γ) and Th2 cytokines (IL-4) in CD4T cells, indicating that the proportion of Th1 and Th2 cells in mice with LGG treatment was in a high equilibrium state compared to the control group. In addition, the IFN-γ/IL-4 ratio was greater than 1 in mice with LGG intervention, suggesting that LGG tends to mediate the Th1 immune response. The results of the present study also showed that LGG upregulated the expression of IL-17 in CD4T cells and regulated the percentage of CD4CD25Foxp3Treg cells in various secondary immunological organs, indicating that LGG may promote the balance of Th-17 and Treg cells.