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Article Publish Status: FREE
Abstract Title:

Prostate and breast cancer cells death induced by xanthohumol investigated with Fourier transform infrared spectroscopy.

Abstract Source:

Spectrochim Acta A Mol Biomol Spectrosc. 2020 Jan 25 ;231:118112. Epub 2020 Jan 25. PMID: 32014658

Abstract Author(s):

Barbara Gieroba, Marta Arczewska, Adrianna Sławińska-Brych, Wojciech Rzeski, Andrzej Stepulak, Mariusz Gagoś

Article Affiliation:

Barbara Gieroba

Abstract:

Fourier Transform Infrared spectroscopy was applied to detect in vitro cell death induced in prostate (PC-3) and breast (T47D) cancer cell lines treated with xanthohumol (XN). After incubation of the cancer cells with XN, specific spectral shifts in the infrared spectra arising from selected cellular components were identified that reflected biochemical changes characteristic for apoptosis and necrosis. Detailed analysis of specific absorbance intensity ratios revealed the compositional changes in the secondary structure of proteins and membrane lipids. In this study, for the first time we examined the changes in these molecular components and linked them to deduce the involvement of molecular mechanisms in the XN-induced death of the selected cancer cells. We showed that XN concentration-dependent changes were attributed to phospholipid ester carbonyl groups, especially in the case of T47D cells, suggesting that XN acts as an inhibitor of cell proliferation. Additionally, we observed distinct changes in the region assigned to the absorption of DNA, which were correlated with a specific marker of cell death and dependent on the XN dose and the type of cancer cells. The microscopic observation and flow cytometry analysis revealed that the decrease in cancer cell viability was mainly related to the induction of necrotic cell death. Moreover, the T47D cells were slightly more sensitive to XN than the PC-3 cells. Considering the results obtained, it can be assumed that apoptosis and necrosis induced by XN may contribute to the anti-proliferative and cytotoxic properties of this flavonoid against cancer cell lines PC-3 and T47D.

Study Type : In Vitro Study

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