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Article Publish Status: FREE
Abstract Title:

Proteomic analysis of the anti-cancer effect of 20S-ginsenoside Rg3 in human colon cancer cell lines.

Abstract Source:

Biosci Biotechnol Biochem. 2009 Apr 23 ;73(4):811-6. Epub 2009 Apr 7. PMID: 19352032

Abstract Author(s):

Seo Young Lee, Geun Tae Kim, Si Hun Roh, Jin-Su Song, Hie-Joon Kim, Soon-Sun Hong, Sung Won Kwon, Jeong Hill Park

Article Affiliation:

Seo Young Lee

Abstract:

Ginseng is a well known herbal medicine in Asia, and ginsenoside Rg3 has anti-cancer and various pharmacological effects. In particular, 20S-ginsenoside Rg3 may increase the anti-proliferative effects of chemotherapy. The authors investigated the mechanism of the anti-proliferative effect of 20S-Rg3 at the protein level in HT29 colon cancer cells. MTT, caspase-3 assays, and flow cytometry analysis were performed to determine cytotoxicity and apoptosis, and proteomic analysis was performed by two-dimensional gel electrophoresis and MALDI-TOF/TOF MS, and a database was used to identify protein changes in 20S-Rg3 treated HT29 cells. The proteins identified included down-regulated Rho GDP dissociation inhibitor, up-regulated tropomyosin1, and annexin5 and glutathione s-transferase p1, which are apoptosis associated proteins. The anti-proliferative mechanism of 20S-Rg3 was found to be involved in mitotic inhibition, DNA replication, and repair and growth factor signaling. The findings of this study suggest that the cytotoxicity of 20S-Rg3 in colon cancer is dependent on several mechanisms, including apoptosis.

Study Type : In Vitro Study

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