Pterostilbene exhibits chemopreventive activity in a rat liver cell line. - GreenMedInfo Summary
Pterostilbene from Vitis coignetiae protect H2O2-induced inhibition of gap junctional intercellular communication in rat liver cell line.
Food Chem Toxicol. 2009 Feb;47(2):404-9. Epub 2008 Dec 7. PMID: 19101604
Food Function Research Center, Food Convergence Technology Division, Korea Food Research Institute, Seongnam, Baekhyun-Dong, Bundang-Ku, Sungnam-Si, Gyeonggi-Do 463-746, Republic of Korea. [email protected]
In this study, we identified various stilbenoids derived from Vitis coignetiae and investigated the protective effect of the main component, pterostilbene, against the inhibition of gap junctional intercellular communication (GJIC) induced by H(2)O(2) in WB-F344 rat liver epithelial cells. We analyzed seven kinds of stilbenoids, pterostilbene, astringin, piceid, vitisin, rhaponticin, resveratrol, and rhapontigenin, using DAD/UV HPLC. Total stilbenoid content was 127.37+/-19.29 mg/100g dry weight. Pretreatment with 0.5, 1.0, and 5.0 microM pterostilbene for 24h was shown to recover GJIC blocked by 500 microM H(2)O(2). Pretreatment with pterostilbene prevented the inhibition of GJIC via the down-regulation of connexin43 phosphorylation by the inactivation of ERK1/2 and p38 MAP kinase. Our results suggest that pterostilbene may be a functional chemopreventative agent and that dietary exposure of pterostilbene would be helpful for improving health.
