Puerarin induces apoptosis in A549 cells. - GreenMedInfo Summary
[Puerarin induces apoptosis in A549 cells].
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2017 May 8 ;33(5):466-469. PMID: 29926595
OBJECTIVE: To explore the effect of puerarin induced apoptosis in human non-small cell lung canner A549 cells and its possible mechanisms.
METHODS: A549 cells were cultivated in vitro. When 80%~90% confluence was reached, they were treated with puerarin in different concentration (60、120、240 μg/ml). The effects on cell apoptpsis were examined by CCK-8 colorimetry. Characteristic morphological changes of apoptosis were observed by AO/EB staining. The apoptosis of A549 cells were detected by flow cytometry analysis. The proteins level of Apelin and APJ were tested by Westernblot.
RESULTS: CCK-8 test showed that puerarin could inhibit the proliferation of A549 cells in a time and concentration dependent manner. Flow cytometry analysis indicated that puerarin could make A549 cells apoptosis. Compared with the A549 cells group, the protein expression of Apelin/APJ was decreased in puerarin groups.
CONCLUSIONS: Puerarin induces apoptosis in A549 cells, and its mechanism may through the regulation of Apelin/APJ expression.