Puerarin promoted proliferation and differentiation of dopamine-producing cells in Parkinson's animal models.
Biomed Pharmacother. 2018 Oct ;106:1236-1242. Epub 2018 Jul 20. PMID: 30119192
BACKGROUND: Parkinson's disease (PD) is caused by the gradual loss of dopamine-producing cells in the brain. This study evaluated the potential neuroprotective role of puerarin (PR) on dopamine (DA)-producing cells in vitro and in vivo.
METHOD: In vitro, the effects of PR on proliferation and differentiation and DA releases of mesenchymal stem cells (MSCs) were assayed by CCK-8, flow cytometry, real-time PCR and ELISA respectively. Then the differentiated cells were labeled with enhanced green fluorescent protein (EGFP) and administrated into PD animal models induced by 6-OHDA. The proliferation and differentiation of labeled cells were identified by fluorescence microscopy and immunostaining.
RESULTS: In vitro, after being treated with different concentrations of PR for 1 week, the TUJ1, TH and DAT protein and mRNA expression and DA releases increased significantly. In vivo, after transplantation of PR-treated DA-producing cells, the symptoms of PD improved significantly from the second week after transplantation; more transplanted cells survived and migrated to wider region along injection line; more transplanted cells proliferated and differentiated into THcells; more DA was detected in the striatum during 6 weeks' observation.
CONCLUSION: The results suggest that PR promote DA neuron survival, proliferation and differentiation.