Anti-Colitic Effect of Purple Carrot on Dextran Sulfate Sodium (DSS)-Induced Colitis in C57BL/6J Mice.
Prev Nutr Food Sci. 2018 Mar ;23(1):77-83. Epub 2018 Mar 31. PMID: 29662851
The anti-colitic effect of purple carrot (PC) on 2% dextran sulfate sodium (DSS)-induced colitis in C57BL6/J mice was compared with those of yellow carrot (YC), beet (BT), and red cabbage (RC). Component analysis showed that PC contained cyanidin-3-xyloglucoside, cyanidin-3-xylosyl(sinapoly-glucosyl)galactoside, cyanidin-3-xylosyl(feruloylglucosyl) galactoside, and cyanidin-3--(6--glycosyl-2--xylosylgalactoside). PC diet (5% in AIN 93G diet) strongly reduced DSS-induced colon shortening and inflammatory cell infiltration in mice, followed by RC, BT, and YC diets. Treatment with PC reduced serum levels of pro-inflammatory cytokines such as tumor necrosis factor-α and interleukin-6 as well as reduced mRNA expression in colon tissue of colitis mice in comparison with other treatments. In addition, PC treatment inhibited colonic mRNA expression of inflammatory factors such as inducible nitric oxide synthase and cyclooxygenase-2 in mice. These results suggestthat PC can attenuate the inflammatory reaction in mice with DSS-induced colitis, probably due to the anthocyanins in PC.