Pycnogenol may increase the cisplatin cytotoxicity in HeLa cells at nongenotoxic doses. - GreenMedInfo Summary
AnStudy on the Interactions of Pycnogenolwith Cisplatin in Human Cervical Cancer Cells.
Turk J Pharm Sci. 2020 Feb ;17(1):1-6. Epub 2020 Feb 19. PMID: 32454754
Objectives: In the treatment of cancer, it is intended to increase the anticancer effect and decrease cytotoxicity using various plant-derived phenolic compounds with chemotherapeutic drugs. Pycnogenol(PYC), a phenolic compound, has been the subject of many studies. Since the mechanisms of the interactions of PYC with cisplatin need to be clarified, we aimed to determine the effects of PYC on cisplatin cytotoxicity in human cervix cancer cells (HeLa) and to evaluate the genotoxicity of PYC.
Materials and Methods: The cytotoxicity of cisplatin and PYC was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in HeLa cells for 24 h and 48 h. The effect of PYC against oxidative DNA damage was evaluated using the comet assay.
Results: The ICvalues of cisplatin were 22.4μM and 12.3 μM for 24 h and 48 h, respectively. The ICvalues of PYC were 261μM and 213 μM for 24 h and 48 h, respectively. For 24 h exposure, PYC significantly reduced the ICvalue of cisplatin at the selected concentrations (15.6-500μM). For 48 h exposure, PYC did not change the cytotoxicity of cisplatin at concentrations between 15.6 and 125 μM, but significantly reduced it at concentrations of 250 μM and 500 μM. PYC alone did not induce DNA damage at concentrations of 10 μM or 25 μM; however, it significantly induced DNA damage at higher concentrations (50-100 μM). It also significantly reduced HO-induced DNA damage at all concentrations studied (10-100μM).
Conclusion: Our results suggest that PYC may increase the cisplatin cytotoxicity in HeLa cells at nongenotoxic doses. The results might contribute to the anticancer effect of cisplatin with PYC in cervical carcinoma, but in order to confirm this result furtherstudies with cancer cell lines andstudies are needed.