Quercetin is an effective inhibitor of quorum sensing, biofilm formation and virulence factors in Pseudomonas aeruginosa.
J Appl Microbiol. 2016 Jan 25. Epub 2016 Jan 25. PMID: 26808465
AIMS: The study aimed to perform a systematic investigation of the effects of quercetin on biofilm formation and virulence factors in Pseudomonas aeruginosa.
METHODS AND RESULTS: The P. aeruginosa strain PAO1 was selected as the test strain. The results indicated that quercetin did not impact the growth of PAO1 as determined by MIC and growth curve analysis. However, this compound significantly inhibited (P<0.05) biofilm formation and production of virulence factors including pyocyanin, protease and elastase at a lower concentration than those for most previously reported plant extracts and substances. Considering the central role of quorum sensing (QS) in the regulation of biofilm and virulence factor, we further detected the transcriptional changes associated with QS and found that the expression levels of lasI, lasR, rhlI and rhlR were significantly reduced (P<0.05) by 34%, 68%, 57% and 50%, respectively, in response to 16μg ml(-1) quercetin.
CONCLUSIONS: This study indicated that quercetin is an effective inhibitor of biofilm formation and virulence factors in P. aeruginosa.
SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first study to demonstrate that quercetin is an effective inhibitor of QS, biofilm formation and virulence factors in P. aeruginosa. Furthermore, quercetin might have potential in fighting biofilm-related infections. This article is protected by copyright. All rights reserved.