Abstract Title:

Glyphosate-induced stiffening of HaCaT keratinocytes, a Peak Force Tapping study on living cells.

Abstract Source:

J Struct Biol. 2012 Feb 17. Epub 2012 Feb 17. PMID: 22369932

Abstract Author(s):

Celine Heu, Alexandre Berquand, Celine Elie-Caille, Laurence Nicod

Article Affiliation:

University of Franche-Comte, Laboratoire de Biologie Cellulaire, EA4267, IFR133, UFR des Sciences Medicales&Pharmaceutiques, 19 rue Ambroise Pare, 25030 Besancon cedex, France; University of Franche-Comte, Clinical&Innovation Proteomic Platform (CLIPP), Institut FEMTO-ST, UMR 6174 CNRS, 25030 Besancon cedex, France.


The skin is the first physiological barrier, with a complex constitution, that provides defensive functions against multiple physical and chemical aggressions. Glyphosate is an extensively used herbicide that has been shown to increase the risk of cancer. Moreover there is increasing evidence suggesting that the mechanical phenotype plays an important role in malignant transformation. Atomic force microscopy (AFM) has emerged within the last decade as a powerful tool for providing a nanometer-scale resolution imaging of biological samples. Peak Force Tapping (PFT) is a newly released AFM-based investigation technique allowing extraction of chemical and mechanical properties from a wide range of samples at a relatively high speed and a high resolution. The present work uses the PFT technology to investigate HaCaT keratinocytes, a human epidermal cell line, and offers an original approach to study chemically-induced changes in the cellular mechanical properties under near-physiological conditions. These experiments indicate glyphosate induces cell membrane stiffening, and the appearance of cytoskeleton structures at a subcellular level, for low cytotoxic concentrations whereas cells exposed to IC50 (inhibitory concentration 50%) treatment exhibit control-like mechanical behavior despite obvious membrane damages. Quercetin, a well-known antioxidant, reverses the glyphosate-induced mechanical phenotype.

Study Type : In Vitro Study
Additional Links
Problem Substances : Glyphosate : CK(716) : AC(241)

Print Options

Key Research Topics

Sayer Ji
Founder of GreenMedInfo.com

Subscribe to our informative Newsletter & get Nature's Evidence-Based Pharmacy

Our newsletter serves 500,000 with essential news, research & healthy tips, daily.

Download Now

500+ pages of Natural Medicine Alternatives and Information.

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2020 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.