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Abstract Title:

EGCG enhances cancer cells sensitivity underCoγ radiation based on miR-34a/Sirt1/p53.

Abstract Source:

Food Chem Toxicol. 2019 Sep 5 ;133:110807. Epub 2019 Sep 5. PMID: 31494133

Abstract Author(s):

Qiaozhen Kang, Xiaomiao Zhang, Nana Cao, Chen Chen, Juanjuan Yi, Limin Hao, Yizhi Ji, Xin Liu, Jike Lu

Article Affiliation:

Qiaozhen Kang

Abstract:

Ionizing radiation (IR) resistance and toxicity to normal cells are the main problems in radiotherapy for cancer. In this study, we demonstrated that epigallocatechin gallate (EGCG) could inhibit effectively IR-induced damage to mouse normal hepatic cells AML-12, and improve dramatically the radiosensitivity of mouse hepatoma cells H22 toCoγ. In addition, the different effects of EGCG and underlying molecular mechanisms based on microRNA-34a (miR-34a) and apoptosis-related proteins were investigated by cells viability analysis, quantitative realtime PCR (qRT-PCR), Western blot and cells transfection. The results indicated EGCG playedthe key role of radiosensitization on H22 cells by activating the miR-34a/Sirt1/p53 signaling pathway. Besides, EGCG could down-regulate the expression of anti-apoptotic protein Bcl-2, and up-regulate the expression of pro-apoptotic proteins Bax and Caspase-3 in H22 cells. Interestingly, EGCG showed contrary results on AML-12 cells. Therefore, radiation protection and radiosensitization of EGCG were associated with apoptosis regulated by miR-34a/Sirt1/p53 signaling pathway.

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