Regulatory Effect ofBmb6 on Gut Barrier Functions in Experimental Colitis.
Foods. 2020 Jul 2 ;9(7). Epub 2020 Jul 2. PMID: 32630643
The integrity of gut barrier functions is closely associated with the pathogenesis of colitis. It is speculated thatBmb6 alleviates colitis by improving the tight junction (TJ) of the inflamed intestinal epithelial layer. In the present study, the regulatory effects ofBmb6 on the TJ barrier to ameliorate colitis-symptoms were investigated. Preliminary screening showed thatBmb6 exhibited strong acid and bile acid tolerance, along with antioxidants andβ-galactosidase activities. In a 14-day dextran sulfate sodium (DSS)-induced colitis mouse model, treatment withBmb6 significantly decreased in the disease activity index score. In addition, histological analyses showed that treatment withBmb6 protected the structural integrity of the intestinal epithelial layer and mucin-secreting goblet cells from DSS-induced damage, with only slight infiltration of immune cells. Interestingly, western blotting analyses showed that the expression of the TJ protein, zona occluden-1, was restored in Bmb6-treated mice, but not in DSS-induced mice. Consistently, the gene expression of inflammatory cytokines (tumor necrosis factor-α and interferon-γ) was also suppressed in the Bmb6-treated mice. Hence, our findings suggest that suppression of inflammatory conditions enhanced expression of TJ protein, ZO-1, or vice versa, contributing to a colitis-ameliorating effect inBmb6.