The effects of cinnamaldehyde on acute or chronic stress-induced anxiety-related behavior and locomotion in male mice.
Stress. 2019 May ;22(3):358-365. Epub 2019 Feb 26. PMID: 30806129
Anxiety and stress are considered as universal psychiatric exhibitions of the present societies and lifestyles. Several experiments have been conducted to examine natural anxiolytic agents to find out an alternative to synthetic anxiolytic drugs. The present study investigated the anxiolytic effects of cinnamaldehyde (Cin) on mice behavior in the elevated plus maze (EPM) and open field (OF) tests. Sixty male Swiss mice, weighing 20-30 g, were divided into six groups including: acute stress + mazola oil; chronic stress + oil; acute stress + Cin (20 mg/kg); chronic stress + Cin; non-stress + oil; and non-stress + Cin groups. The groups were administered for seven days (once a day). The acute stress + Cin group showed a meaningful rise in the percentage of entries into the open arms compared to the acute stress + oil group (p <.05). The percentage of time spent in the open arms in the chronic stress + Cin group was significantly higher compared to the chronic stress + oil group (p < .01). The percentage of entries into the open arms increased significantly (p < .01) in the chronic stress + Cin group in comparison with the chronic stress + oil group. The Cin treated groups showed significant increases in the time spent in the center area and in the number of entries into the center area compared with the oil treated groups in OF test. The numberof entries into the arms (total activity), as well as locomotor activity was not significant among groups. The results of the present study indicated that Cin, as a natural product, might have anxiolytic effects in mice behavior in the EPM and OF tests. Lay summary The results demonstrated that the administration of cinnamaldehyde (Cin) produced anxiolytic effects in mice. Natural antioxidant products have been reported effective for anxiety. Synthetic medications have notable adverse effects. Therefore, these natural substances with broad therapeutic applicability are able to reduce anxiety-related behavior with rare side effects. According to the results, Cin could decrease anxiety-related behavior in mice.