Efficacy and Safety ofBoiss Essential Oil against Acute Toxoplasmosis in Mice.
Iran J Parasitol. 2020 Jan-Mar;15(1):22-30. PMID: 32489372
Background: Broad spectrums of pharmacological properties, including antimicrobial activity have been attributed toBoiss (Laminaceae). The in vivo efficacy and safety ofessential oil (ZM-EO) were evaluated against acute toxoplasmosis caused by(Sarcocystidae) in mice.
Methods: (aerial parts) was obtained from the rural districts of Kerman city (Kerman Province) Southwestern Iran, in May of 2016. Male NMRI mice were orally treated with normal saline (control group) and ZM-EO at the doses of 0.2 and 0.4 mL/kg once a day for 14 d (8 mice in each group)On the 15day, the mice were infected with 104 tachyzoites ofRH strain by intraperitoneal route. The mortality rate and parasite load were determined in the infected mice. Additionally, 24 mice were applied to examine the sub-acute toxicity of ZM-EO at the above doses after treatment during 14 d.
Results: GC/MS analysis displayed that the key constituents were thymol (45.4%), carvacrol (23%) and-cymene (10.6%), respectively. Overall, 100% mortality was observed on the 8and 9days in treated mice with the concentrations of 0.2 and 0.4 mL/kg, respectively. The mean number of tachyzoites in the mice treated with 0.2 and 0.4 mL/kg of ZM-EO were 189×10and 76×10cell/mL, respectively, meaningfully (<0.05) reduced compared with the control mice. Results also demonstrated that ZM-EO had no important toxicity on mice.
Conclusion: The results demonstrated the efficacy of ZM-EO against acute toxoplasmosis. Nevertheless, supplementary surveys are mandatory to examine its precise effects, mainly immunomodulatory effect on toxoplasmosis.