Antioxidant effects of() on cyclophosphamide-induced testicular injury in rats.
Vet Res Forum. 2018 ;9(1):35-41. Epub 2018 Mar 15. PMID: 29719662
Cyclophosphamide (CP) is known to reduce fertility. The protective effects of(SP) against CP-induced testicular toxicity were investigated. Male Wistar rats were categorized into eight groups (n = 7). Four groups of rats were administered CP at a dose of 5 mg in 5 mL distilled water kgper day orally. Two of these groups were received SP (500 and 1000 mg kgper day) orally after CP administration. One of these groups was also received vitamin E (100 mg kgper day) intraperitoneally. A vehicle treated control group, two SP control groups (500 and 1000 mg kgper day) and a vitamin E control group were also included. Body and testes weights, sperm count, serum levels of glutathione peroxidase (GPx), malondialdehyde (MDA), histological and histomorphometric alternations in testes were investigated after four weeks. The CP-treated group exhibited significant decreases in the body and testes weights and spermatogenic activities. Several histological alterations were observed in this group. The CP treatment caused a significant reduction in sperm count, in serum level of GPx, as well increased serum concentration of MDA. The SP co-administration caused an increase in GPx serum level, a decrease in MDA serum level and improvements in histological and histomorphometric alternations. Vitamin E co-treatment showed partial recovery in above-mentioned parameters. These results suggest that SP due to a reduction in oxidative stress has more effective protection against CP-induced reproductive damages in rat than vitamin E.