Resveratrol could depress CCSC proliferation in a time-and dose-depended manner, arrest cell cycle in the G0/G1 phase and promote cell apoptosis. - GreenMedInfo Summary

Objective To investigate the effect of resveratrol (Res) on the proliferation, apoptosis and immunogenicity of colorectal cancer stem cells (CCSCs). Methods The CCSCs were induced from colon cancer cell line HCT116 in serum-free medium (SFM). The expressions of CD133 and CD44 were detected by flow cytometry to identify CCSCs. After treatment with Res at (12.5-200.0)μmol/L, the effect of Res on CCSC proliferation was detected by MTT assay ; cell apoptosis was examined by flow cytometry combined with annexin V-FITC/PI staining; cell cycle and the expression of major histocompatibility complex class I-related chain A and B (MICA/B) were assessed by flow cytometry. Results HCT116 cells formed cancer stem cell spheres in SFM. The proportion of CD133(+) cells in cell spheres was (91.07±1.79)%, and CD44(+) cells was (90.33±1.78)%. Compared with control groups, Res significantly inhibited CCSC proliferation in a time- and dose-depended manner. After treatmentwith Res for 48 hours, the proportion of cells increased in the G0/G1 phase and decreased in the S phase, both in a dose-depended manner. Apoptosis rate of CCSCs and the expression of MICA/B were raised with the increasing concentration of Res. Conclusion CCSCs were successfully induced from HCT116colon cancer cell line. Res could depress CCSC proliferation in a time-and dose-depended manner, arrest cell cycle in the G0/G1 phase and promote cell apoptosis. Res could up-regulate the expression of MICA/B in CCSCs and enhance cell immunogenicity.