Abstract Title:

Modulation of intracellular glutathione affects adipogenesis in 3T3-L1 cells.

Abstract Source:

Carcinogenesis. 2010 Sep 1. Epub 2010 Sep 1. PMID: 21136464

Abstract Author(s):

Paola Vigilanza, Katia Aquilano, Sara Baldelli, Giuseppe Rotilio, Maria Rosa Ciriolo

Article Affiliation:

University of Rome "Tor Vergata" - Department of Biology - Via della Ricerca Scientifica, 1 - 00133 Rome (Italy).

Abstract:

Impairment of redox homeostasis has been extensively associated with obesity, as a consequence of the chronic inflammatory state present in overweight subjects. Deregulation of glutathione (GSH), the most important non-enzymatic intracellular antioxidant, induces insulin resistance in mature adipocytes, but data are lacking about its effects on adipogenesis. In this report we demonstrate that during adipogenesis of 3T3-L1 cells the GSH/GSSG ratio decreases, shifting redox status towards oxidizing conditions. Moreover, we demonstrate that inhibition of GSH synthesis, obtained by treatment with L-buthionine-sulfoximine (BSO), enhances C/EBPβ LAP/LIP ratio and PPARγ expression during mitotic clonal expansion (MCE) stimulating adipogenesis. On the contrary, GSH ethyl ester (GSHest) supplementation completely abrogates this process also in the presence of BSO. GSH decrement during the first 24h of adipogenesis is sufficient to induce higher triglyceride accumulation in differentiated adipocytes with respect to control, whereas GSHest treatment inhibits lipid droplets formation. We further demonstrate that Resveratrol (RV) could exert anti-adipogenic properties also by increasing GSH content through γ-glutamyl-cysteine ligase (GCL) induction. Overall data indicate that in pre-adipocytes the decrease of GSH accelerates adipogenesis, suggesting that the use of agents able to maintain GSH redox status in adipose tissue, such as RV, could be promising in stopping the lipogenic loop of obesity. © 2010 Wiley-Liss, Inc.

Study Type : In Vitro Study
Additional Links
Pharmacological Actions : Anti-Adipogenic : CK(110) : AC(52)

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