Resveratrol has a protective effect on neonatal rat cardiomyocyte lesion induced by hypoxia. - GreenMedInfo Summary
[Protective effects of resveratrol on neonatal rat cardiomyocyte lesion induced by hypoxia].
Zhongguo Zhong Yao Za Zhi. 2010 Jan;35(1):94-8. PMID: 20349725
Institute of Chinese Minority Traditional Medicine Minzu University of China, Beijing 100081, China. firstname.lastname@example.org
OBJECTIVE: To investigate the effects of resveratrol (Res) on neonatal rat cardiomyocyte lesion induced by hypoxia.
METHOD: The cardiomyocyte of neonatal rats were cultured in vitro and the model of cardiomyocyte hypoxia was established. The cardiomyocyte vitalities were determined by MTT assay, the HIF-1alpha expression levels in myocardial cells was detected by immunohistochemical, the activities of peroxidase (GSH-Px) and lactate dehydrogenase (LDH) were measured as well.
RESULT: After the administration of hypoxia for 24 hours, the HIF-1alpha expression in myocardial cells was significantly increased. The LDH level in the culture medium was increased from (93.07 +/- 15.84) U x L(-1) to (750.77 +/- 181.51) U x L(-1) (P<0.01). The intracellular GSH-Px activity was decreased from (46.96 +/- 8.36) U x mL(-1) to (27.13 +/- 4.76) U x mL(-1) (P<0.05). Res 25, 50 and 75 micromol x L(-1) could dose-dependently inhibit the raising of the HIF-1alpha expression in myocardial cells induced by hypoxia. The LDH activities were decreased dose-dependently to (486.17 +/- 69.97), (189.43 +/- 32.07), (155.34 +/- 29.57) U x L(-1), respectively (P<0.05 or P<0.01). The GSH-Px activities were increased dose-dependently (33.55 +/- 6.34), (37.67 +/- 6.73), (41.44 +/- 7.91) U x mL(-1) (P<0.05 or P<0.01).
CONCLUSION: Res has a protective effect on neonatal rat cardiomyocyte lesion induced by hypoxia.