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Abstract Title:

In Vitro Anticancer MCF-7, Anti-Inflammatory, and Brine Shrimp Lethality Assay (BSLA) and GC-MS Analysis of Whole Plant Butanol Fraction of(WBFRR).

Abstract Source:

Biomed Res Int. 2019 ;2019:3264846. Epub 2019 Jun 23. PMID: 31341895

Abstract Author(s):

Jahangir Khan Achakzai, Muhammad Anwar Panezai, Muhammad Ayub Kakar, Abdul Manan Kakar, Shahabuddin Kakar, Javed Khan, Nazima Yousaf Khan, Inayatullah Khilji, Ajab Khan Tareen

Article Affiliation:

Jahangir Khan Achakzai

Abstract:

In this study, GC-MS analysis has shown that whole plant butanol fraction of(WBFRR) comprises of 21 compounds which exhibited anticancer (MCF-7) activity having ICvalue of 36.01± 0.26. MTT assay (MCF-7), Oxidative Burst assay using chemiluminescence technique, and B-Hatching techniques were the methods used for anticancer MCF-7, anti-inflammatory, and Brine Shrimp Lethality Assay (BSLA). GC-MS was used for structural elucidation. Whole plant methanol extract of(WMERR), whole plant n-hexane fraction of(WHFRR), and whole plant aqueous fraction of(WAFRR) were inactive against anticancer (MCF-7) cell line. Whole plant methanol extract of(WMERR), whole plant aqueous fraction of(WAFRR) and whole plant butanol fraction of(WBFRR) showed anti-inflammatory activity on ROS having ICvalue of 23.2±1.9, 24.2±2.7 and 12.0±0.6. Whole plant butanol fraction of(WBFRR) showed Brine Shrimp Lethality with LD50 693.302 while whole plant methanol extract of(WMERR) and whole plant aqueous fraction of(WAFRR) showed high lethality at highest concentration. This study revealed that whole plant butanol fraction of(WBFRR) exhibited significant anticancer (MCF-7) activity. In the near future, the constituent of whole plant butanol fraction of(WBFRR) can be the alternative drug against MCF-7 cell line with least toxicity and side effects.

Study Type : In Vitro Study

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