Rosmarinic Acid/ Blue Light Combination Treatment Inhibits Head and Neck Squamous Cell Carcinoma.
Anticancer Res. 2020 Feb ;40(2):751-758. PMID: 32014917
Christi N Waer
BACKGROUND/AIM: This study investigated a novel combined therapy of rosmarinic acid (RA)/blue light on head and neck squamous cell carcinoma (HNSCC) cell proliferation in vitro.
MATERIALS AND METHODS: HNSCC cells were exposed to BL (500 mW/cm) for 90 s, and incubated with 80μg/ml RA for 1 hour. Cell viability was determined after 24 h using WST-1 assay. Western blot was used to detect treatment-induced changes in epidermal growth factor receptor (EGFR) activation. Hydrogen peroxide (HO) and nitric oxide levels were quantified using CM-HDCFH-DA assays. Apoptosis was assessed using Annexin V/PI staining and flow cytometry.
RESULTS: RA/blue light treatment resulted in a significant reduction in cell viability, EGFR activation and HOlevels in all HNSCC cell lines. However, no significant changes in NO production or apoptosis induction were found.
CONCLUSION: RA/blue light effectively decreased HNSCC cell proliferation through reduction in EGFR activation and HOproduction, and not via induction of apoptosis.