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Abstract Title:

Sensitization of MDA-MBA231 breast cancer cell to docetaxel by myricetin loaded into biocompatible lipid nanoparticles via sub-G1 cell cycle arrest mechanism.

Abstract Source:

Naunyn Schmiedebergs Arch Pharmacol. 2019 Aug 2. Epub 2019 Aug 2. PMID: 31372697

Abstract Author(s):

Nazila Fathi Maroufi, Vahid Vahedian, Seyed Ali Miresmaeili Mazrakhondi, Wesam Kooti, Hosein Ajami Khiavy, Roya Bazzaz, Fatemeh Ramezani, Seyed Mohammadbagher Pirouzpanah, Marjan Ghorbani, Maryam Akbarzadeh, Hamed Hajipour, Saeed Ghanbarzadeh, Mehdi Sabzichi

Article Affiliation:

Nazila Fathi Maroufi

Abstract:

The harmful dose-dependent side effects of chemotherapy drugs have caused the discovery of novel perspective to evaluate chemotherapy protocols. In this study, the potential application of Compritol was investigated as a major scaffold into nanostructured lipid careers to highlight myricetin efficiency in treatment of breast cancer cells along with codelivery of docetaxel (DXT). Characterization of myricetin-loaded NLCs was carried out by measuring the particle size and zeta potential, using the scanning electron microscopy. MTT, DAPI staining, flow cytometric, and RT-PCR (real-time) assays were used to recognize novel formulation behavior on cell cytotoxicity as well as recognizing molecular mechanism of formulation concerning apoptosis phenomenon. Myricetin-loaded NLCs reduced the cell viability from 50 ± 2.3 to 40 ± 1.3% (p < 0.05). Percentage of apoptosis improved with combination treatment of myricetin-loaded NLCs and DXT in the MDA-MBA231 breast cancer cells. Expression of antiapoptotic genes (survivin, Cyclin B1, and Mcl1) indicated a significant reduction in factor along with increment in proapoptotic factor Baxand Bid mRNA rates. Overall, our results represented that the NLC delivery system could be a promising strategy to enhance the effect of anticancer agents such as DXT on breast cancer.

Study Type : In Vitro Study

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