Shikonin, vitamin K3 and vitamin K5 inhibit multiple glycolytic enzymes in MCF-7 cells. - GreenMedInfo Summary
Shikonin, vitamin Kand vitamin Kinhibit multiple glycolytic enzymes in MCF-7 cells.
Oncol Lett. 2018 May ;15(5):7423-7432. Epub 2018 Mar 13. PMID: 29725454
Glycolysis is the most important source of energy for the production of anabolic building blocks in cancer cells. Therefore, glycolytic enzymes are regarded as potential targets for cancer treatment. Previously, naphthaquinones, including shikonin, vitamin Kand vitamin K, have been proven to decrease the rate of glycolysis in cancer cells, which is partly due to suppressed pyruvate kinase activity. In the present study, enzymatic assays were performed using MCF-7 cell lysate in order to screen the profile of glycolytic enzymes in cancer cells inhibited by shikonin, vitamin Kand vitamin K, in addition to pyruvate kinase. Results revealed that hexokinase, phosphofructokinase-1, fructose bisphosphate aldolase, glyceraldehyde-3-phosphate dehydrogenase and pyruvate kinase produced in the process of glycolysis were inhibited by shikonin, vitamin Kand vitamin K. The results indicated that shikonin, vitamin Kand vitamin Kare chemical inhibitors of glycolytic enzymes in cancer cells and have potential uses in translational medical applications.