Simultaneous Use of Stimulatory Agents to Enhance the Production and Hypoglycaemic Activity of Polysaccharides fromby Submerged Fermentation.
Molecules. 2019 Dec 2 ;24(23). Epub 2019 Dec 2. PMID: 31810277
This study aimed to determine the effect of applying stimulatory agents to liquid culturedon the simultaneous accumulation of exo-polysaccharides (EPS) and their monosaccharide composition. Different stimulatory agents (VB, VB, betulin and birch extract) were investigated for their effects on active exo-polysaccharides by submerged fermentation of. The mycelial biomass, reducing sugar content, EPS yield andα-glucosidase inhibition rate were determined, and the EPS obtained was analyzed for monosaccharide composition. The results showed that the addition of all the four stimulatory agents could significantly increase the inhibitory activity against α-glucosidase of EPS than the control, whereas EPS from 4 μg/mL VB-containing medium had the best effect with an estimated ICvalue 24.34μg/mL. Among the four stimulatory agents, VBgave maximum production of mycelial biomass and EPS at the concentration of 4μg/mL with a increase of 50.79% and 114.46%, respectively. In addition, betulin had a significant effect on increasing the EPS yield and activity, and birch extract had a significantly stimulatory effect on the mycelial growth and the polysaccharides activity, only slightly worse than VBand VB. Moreover, the addition of different stimulatory agents changed the monosaccharide composition of polysaccharides, which had a correlation with polysaccharide activity.