Abstract Title:

Stannous chloride mediates single strand breaks in plasmid DNA through reactive oxygen species formation.

Abstract Source:

Toxicol Lett. 1999 Nov 22;110(3):129-36. PMID: 10597023

Abstract Author(s):

F J Dantas, M O Moraes, J C de Mattos, R J Bezerra, E F Carvalho, M B Filho, A Caldeira de Araújo

Article Affiliation:

Departamento de Biofisica e Biometria, Instituto de Biologia Roberto Alcântara Gomes, UERJ, Rio de Janeiro, Brazil.

Abstract:

Stannous ion (Sn) has been employed in nuclear medicine and in food industry. We described that Stannous Chloride (SnCl2) inactivation effect in Escherichia coli is mediated by a Fenton-like reaction. The effect of SnCl2 was studied through: (i) the alteration of plasmid topology in neutral and acidic pH by gel electrophoresis; and (ii) the transformation efficiency of an wild type E. coli strain. Treatment of plasmid DNA pUC 9.1 with SnCl2, at pH 7.4, results in DNA single-strand breaks (SSB), in a dose-dependent manner. Addition of sodium benzoate partly inhibited the DNA damage, while EDTA completely abolishes DNA-SSB. Furthermore, the ability of the plasmid to transform E. coli was reduced. At pH 1.3, SnCl2 exerts a protective effect on plasmid against HCI depurination. Our results suggest the generation of ROS, such as *OH by a Fenton-like reaction, close to the site of the lesions due to a possible complexation of stannous ion to DNA.

Study Type : In Vitro Study
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